SUCCESS! :D I used the i386 libmyodbc.so and pointed odbcinst.ini to it in my driver. This still gave me an error with one of libmyodbc.so's dependencies, libmysqlclient_r.so.15, which is part of the libmysqlclient15off package. So I downloaded that package as well in its i386 version, copied its binary contents to a folder, and pointed to that folder using export LD_LIBRARY_PATH=thatfolder so libmyodbc.so could locate it. Then I tried my WINE application and it successfully connected and returned a record! SO TO SUM UP, AFTER A REGULAR unixODBC -> WINE INSTALL, WHAT YOU SHOULD DO TO GET IT WORKING UNDER AMD64 IS: - Install package ia32-libs (it should be already installed with WINE, though) - Edit /etc/bash.bashrc - Add the following line at the end of the file: export LIB_ODBC_DRIVER_MANAGER=/emul/ia32-linux/usr/lib/libodbc.so.1.0.0 - Download the i386 libmyodbc package - Extract file libmyodbc.so - Rename it to something like libmyodbc-i368.so - Copy it to /usr/lib/odbc - Edit /etc/odbcinst.ini, and modify the DRIVER line of your driver so it points to the i386 libmyodbc, i.e.: Driver = /usr/lib/odbc/libmyodbc-i386.so - Save odbcinst.ini - Download the i386libmysqlclient15off package - Extract library files (.so) and symlinks to a folder of your choice, i.e.: /usr/lib/odbc/libmysqlclient-i386 - Edit /etc/bash.bashrc - At the end of the file, add the following line: export LD_LIBRARY_PATH=/usr/lib/odbc/libmysqlclient-i386 - Save bash.bashrc and restart Now the driver should work from WINE in AMD64. Thanks felix and hellork, I couldn't have got this working without your help.