Hi,
I want to covert this format into array, as same as to recursively. Please help me.
Expecting favorable suggestion
Thanks in advance
S.Ram
-------------------------------
Pubmed-entry ::= {
pmid 16192638,
medent {
em std {
year 2005,
month 9,
day 29
},
cit {
title {
name "Bovine prion is endocytosed by human enterocytes via the 37
kDa/67 kDa laminin receptor."
},
authors {
names ml {
"Morel E",
"Andrieu T",
"Casagrande F",
"Gauczynski S",
"Weiss S",
"Grassi J",
"Rousset M",
"Dormont D",
"Chambaz J"
},
affil str "UMR505 INSERM/UPMC, 15 rue de l'ecole de Medecine, 75006
Paris, France."
},
from journal {
title {
iso-jta "Am. J. Pathol.",
ml-jta "Am J Pathol",
issn "0002-9440"
},
imp {
date std {
year 2005,
month 10
},
volume "167",
issue "4",
pages "1033-1042",
language "eng",
pubstatus ppublish,
history {
{
pubstatus pubmed,
date std {
year 2005,
month 9,
day 30,
hour 9,
minute 0
}
},
{
pubstatus medline,
date std {
year 2005,
month 12,
day 13,
hour 9,
minute 0
}
},
{
pubstatus other,
date std {
year 2005,
month 9,
day 30,
hour 9,
minute 0
}
}
}
}
},
ids {
pii "167/4/1033",
pubmed 16192638,
other {
db "pmc",
tag str "PMC1603663"
}
}
},
abstract "Some forms of transmissible spongiform encephalopathies result
from oral infection. We have thus analyzed the early mechanisms that could
account for an uptake of infectious prion particles by enterocytes, the major
cell population of the intestinal epithelium. Human Caco-2/TC7 enterocytes
cultured on microporous filters were incubated with different prion strains
and contaminated brain homogenates in the apical compartment. Internalization
of infectious particles was analyzed by Western blotting and
immunofluorescence. We observed internalization by enterocytes of prion
particles from bovine spongiform encephalopathy brain homogenates but not
from mouse-adapted scrapie-strain brain homogenates or purified bovine
spongiform encephalopathy scrapie-associated fibrils. Bovine prion particles
were internalized via endocytosis within minutes of infection and were
associated with subapical vesicular structures related to early endosomes.
The endocytosis of the infectious bovine PrP(Sc) was reduced by preincubating
the cells with an anti-LRP/LR blocking antibody, identifying the 37 kDa/67
kDa laminin receptor (LRP/LR), which is apically expressed in Caco-2/TC7
cells, as the receptor for the infectious prion protein. Altogether, our
results underscore a potential role of enterocytes in the absorption of
bovine prions during oral infection through specific LRP/LR-dependent
endocytosis.",
mesh {
{
term "Animals"
},
{
term "Antibodies, Monoclonal",
qual {
{
subh "metabolism"
}
}
},
{
term "Blotting, Western"
},
{
term "Caco-2 Cells"
},
{
term "Cattle"
},
{
term "Electric Impedance"
},
{
term "Encephalopathy, Bovine Spongiform",
qual {
{
subh "metabolism"
}
}
},
{
mp TRUE,
term "Endocytosis"
},
{
term "Enterocytes",
qual {
{
mp TRUE,
subh "metabolism"
}
}
},
{
term "Fluorescent Antibody Technique, Direct"
},
{
term "Humans"
},
{
term "L-Lactate Dehydrogenase",
qual {
{
subh "secretion"
}
}
},
{
term "Membranes, Artificial"
},
{
term "Microscopy, Confocal"
},
{
term "Molecular Weight"
},
{
term "PrPC Proteins",
qual {
{
subh "genetics"
},
{
subh "metabolism"
}
}
},
{
term "PrPSc Proteins",
qual {
{
subh "genetics"
},
{
subh "metabolism"
}
}
},
{
term "Prions",
qual {
{
subh "genetics"
},
{
mp TRUE,
subh "metabolism"
}
}
},
{
term "Receptors, Laminin",
qual {
{
subh "genetics"
},
{
mp TRUE,
subh "physiology"
}
}
}
},
substance {
{
type nameonly,
name "Antibodies, Monoclonal"
},
{
type nameonly,
name "Membranes, Artificial"
},
{
type nameonly,
name "PrPC Proteins"
},
{
type nameonly,
name "PrPSc Proteins"
},
{
type nameonly,
name "Prions"
},
{
type nameonly,
name "Receptors, Laminin"
},
{
type ec,
cit "1.1.1.27",
name "L-Lactate Dehydrogenase"
}
},
pmid 16192638,
pub-type {
"Journal Article",
"Research Support, Non-U.S. Gov't"
},
status medline
}
}
[Non-text portions of this message have been removed]
