Running on: Gentoo linux-2.6.30-gentoo-r4 gcc x86_64-pc-linux-gnu-4.3.4 wine 1.1.33 jack-audio-connection-kit 0.116.2 jackd gives the following output after testing jack in winecfg Code: jackd 0.116.2 Copyright 2001-2005 Paul Davis and others. jackd comes with ABSOLUTELY NO WARRANTY This is free software, and you are welcome to redistribute it under certain conditions; see the file COPYING for details JACK compiled with System V SHM support. loading driver .. creating alsa driver ... hw:0|hw:0|1024|2|48000|0|0|nomon|swmeter|-|32bit control device hw:0 configuring for 48000Hz, period = 1024 frames (21.3 ms), buffer = 2 periods ALSA: final selected sample format for capture: 32bit integer little-endian ALSA: use 2 periods for capture ALSA: final selected sample format for playback: 32bit integer little-endian ALSA: use 2 periods for playback JACK protocol mismatch (16 vs 24) cannot complete client connection process JACK protocol mismatch (16 vs 24) cannot complete client connection process Jack protocol mismatch (16 vs 24) seems to be the main problem. I've uninstalled and recompiled jack and this still occurs. I've done a revdep-rebuild --library=libjack.so.0 and recompiled wine. What else can be done?