hi there - does anybody have a script/plugin that can be used for 'peak integration' ? ie given a b/w image with 'spots' measure total intensity in a box and substract 'background' ? (ie two boxes need defined, or better make it auto ... ie click in centre abd then decide what's spot and what backgr. within some threshold limits ...) Its basically a little extension to 'Histogram' ... Can be amazingly usefull for folks in my field (mol.biology/biochemistry) where we often have 'peaks' on an image with background and we want to quantify ... Thanks for any help ... Tassos