change /etc/security/limits.conf need reboot or not?

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We have CEntos 5.5 on DELL server.  Due to more and more databases run on same server sometime processes number read max and cause 'resource temporary unavailable".

I plan to change /etc/security/limits.conf value:

oracle          soft    nproc          2047 ==> 4096

Do i need reboot to make it effect?

How to test change is effect?

Thanks.
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